[Sequence characterization and differential expression of a glutathione S-transferase gene vv-gto1 from Volvariella volvacea].
Identifieur interne : 000290 ( Main/Exploration ); précédent : 000289; suivant : 000291[Sequence characterization and differential expression of a glutathione S-transferase gene vv-gto1 from Volvariella volvacea].
Auteurs : Junjie Yan ; Lixian Guo ; Jingjing Zhao ; Baogui XieSource :
- Wei sheng wu xue bao = Acta microbiologica Sinica [ 0001-6209 ] ; 2014.
Descripteurs français
- KwdFr :
- Alignement de séquences (MeSH), Analyse de séquence (MeSH), Données de séquences moléculaires (MeSH), Exons (MeSH), Glutathione transferase (composition chimique), Glutathione transferase (génétique), Glutathione transferase (métabolisme), Introns (MeSH), Phylogenèse (MeSH), Protéines fongiques (composition chimique), Protéines fongiques (génétique), Protéines fongiques (métabolisme), Régulation de l'expression des gènes fongiques (MeSH), Séquence d'acides aminés (MeSH), Volvariella (classification), Volvariella (composition chimique), Volvariella (enzymologie), Volvariella (génétique).
- MESH :
- composition chimique : Glutathione transferase, Protéines fongiques, Volvariella.
- enzymologie : Volvariella.
- génétique : Glutathione transferase, Protéines fongiques, Volvariella.
- métabolisme : Glutathione transferase, Protéines fongiques.
- Alignement de séquences, Analyse de séquence, Données de séquences moléculaires, Exons, Introns, Phylogenèse, Régulation de l'expression des gènes fongiques, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Exons (MeSH), Fungal Proteins (chemistry), Fungal Proteins (genetics), Fungal Proteins (metabolism), Gene Expression Regulation, Fungal (MeSH), Glutathione Transferase (chemistry), Glutathione Transferase (genetics), Glutathione Transferase (metabolism), Introns (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Sequence Alignment (MeSH), Sequence Analysis (MeSH), Volvariella (chemistry), Volvariella (classification), Volvariella (enzymology), Volvariella (genetics).
- MESH :
- chemical , chemistry : Fungal Proteins, Glutathione Transferase.
- chemical , genetics : Fungal Proteins, Glutathione Transferase.
- chemical , metabolism : Fungal Proteins, Glutathione Transferase.
- chemistry : Volvariella.
- classification : Volvariella.
- enzymology : Volvariella.
- genetics : Volvariella.
- Amino Acid Sequence, Exons, Gene Expression Regulation, Fungal, Introns, Molecular Sequence Data, Phylogeny, Sequence Alignment, Sequence Analysis.
Abstract
OBJECTIVE
Based on the analysis of omics data of Volvariella volvacea, a gene encoding glutathione S- transferase (GSTs) named vv-gtol was obtained. To reveal the role of GSTs in the growth and development in edible fungi, the structure, the sequence characters and the expression profile of a GST gene vv-gto1 of Volvariella volvacea were analyzed.
METHODS
ZOOM software was used to map sequencing read (reads) from genome and transcriptome against the splicing sequence of genome, to confirm the complete length and the accuracy of the gene sequence, and to visualize gene structure. The MEGA 5.1 was used to do the multiple sequence alignment and phylogenetic tree analysis. Real time fluorescent quantitative PCR was used to determine the expression levels of vv-gtol at different growth periods of Volvariella volvacea.
RESULTS
The full sequence of vv-gtol covered 2083 bp, containing 11 exons and 10 introns, and encoded a protein with 356 amino acids. 5'UTR was 305 bp which contains one intron region, and 3'UTR was 86bp. Two intron retentions could be recognized during RNA processing, and the transcripts formed by the intron retention could not translate the correct conservative functional domains. The full-length of vv-gtol had more than 50 accurate positioning genome sequencing reads, suggesting that genome sequencing and assembly results are accurate and reliable. The phylogenetic tree showed that GTO1 of Volvariella volvacea belonged to the subclass I of the Omega class of glutathione S-transferase superfamily, and had the closest relationship with GTO1 and GTO2 in Phanerochaete chrysosporium. The analysis of digital gene expression profiling, fluorescence quantitative PCR and proteomics showed that vv-gtol had the highest expression level in the heterokaryotic hyphae.
CONCLUSIONS
This is the first time to obtain a gene encoding glutathione S-transferase from Volvariella volvacea which belongs to Omega class. Our study showed that the gene may play an important role during the special biological functions of heterokaryotic hyphae. This study also suggested that Volvariella volvacea heterokaryotic hyphae in H1521 had stronger resistance ability than other samples. In addition, vv-gto1 could form different alternative splicesome to regulate gene transcription and translation, and ultimately affect the function of the protein.
PubMed: 25803894
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Sequence characterization and differential expression of a glutathione S-transferase gene vv-gto1 from Volvariella volvacea].</title><author><name sortKey="Yan, Junjie" sort="Yan, Junjie" uniqKey="Yan J" first="Junjie" last="Yan">Junjie Yan</name></author><author><name sortKey="Guo, Lixian" sort="Guo, Lixian" uniqKey="Guo L" first="Lixian" last="Guo">Lixian Guo</name></author><author><name sortKey="Zhao, Jingjing" sort="Zhao, Jingjing" uniqKey="Zhao J" first="Jingjing" last="Zhao">Jingjing Zhao</name></author><author><name sortKey="Xie, Baogui" sort="Xie, Baogui" uniqKey="Xie B" first="Baogui" last="Xie">Baogui Xie</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:25803894</idno><idno type="pmid">25803894</idno><idno type="wicri:Area/Main/Corpus">000258</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000258</idno><idno type="wicri:Area/Main/Curation">000258</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000258</idno><idno type="wicri:Area/Main/Exploration">000258</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Sequence characterization and differential expression of a glutathione S-transferase gene vv-gto1 from Volvariella volvacea].</title><author><name sortKey="Yan, Junjie" sort="Yan, Junjie" uniqKey="Yan J" first="Junjie" last="Yan">Junjie Yan</name></author><author><name sortKey="Guo, Lixian" sort="Guo, Lixian" uniqKey="Guo L" first="Lixian" last="Guo">Lixian Guo</name></author><author><name sortKey="Zhao, Jingjing" sort="Zhao, Jingjing" uniqKey="Zhao J" first="Jingjing" last="Zhao">Jingjing Zhao</name></author><author><name sortKey="Xie, Baogui" sort="Xie, Baogui" uniqKey="Xie B" first="Baogui" last="Xie">Baogui Xie</name></author></analytic><series><title level="j">Wei sheng wu xue bao = Acta microbiologica Sinica</title><idno type="ISSN">0001-6209</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Exons (MeSH)</term><term>Fungal Proteins (chemistry)</term><term>Fungal Proteins (genetics)</term><term>Fungal Proteins (metabolism)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Glutathione Transferase (chemistry)</term><term>Glutathione Transferase (genetics)</term><term>Glutathione Transferase (metabolism)</term><term>Introns (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Phylogeny (MeSH)</term><term>Sequence Alignment (MeSH)</term><term>Sequence Analysis (MeSH)</term><term>Volvariella (chemistry)</term><term>Volvariella (classification)</term><term>Volvariella (enzymology)</term><term>Volvariella (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences (MeSH)</term><term>Analyse de séquence (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Exons (MeSH)</term><term>Glutathione transferase (composition chimique)</term><term>Glutathione transferase (génétique)</term><term>Glutathione transferase (métabolisme)</term><term>Introns (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Protéines fongiques (composition chimique)</term><term>Protéines fongiques (génétique)</term><term>Protéines fongiques (métabolisme)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Volvariella (classification)</term><term>Volvariella (composition chimique)</term><term>Volvariella (enzymologie)</term><term>Volvariella (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fungal Proteins</term><term>Glutathione Transferase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term><term>Glutathione Transferase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fungal Proteins</term><term>Glutathione Transferase</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Volvariella</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Volvariella</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Glutathione transferase</term><term>Protéines fongiques</term><term>Volvariella</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Volvariella</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Volvariella</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Volvariella</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glutathione transferase</term><term>Protéines fongiques</term><term>Volvariella</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glutathione transferase</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Exons</term><term>Gene Expression Regulation, Fungal</term><term>Introns</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Alignment</term><term>Sequence Analysis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Analyse de séquence</term><term>Données de séquences moléculaires</term><term>Exons</term><term>Introns</term><term>Phylogenèse</term><term>Régulation de l'expression des gènes fongiques</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>OBJECTIVE</b></p><p>Based on the analysis of omics data of Volvariella volvacea, a gene encoding glutathione S- transferase (GSTs) named vv-gtol was obtained. To reveal the role of GSTs in the growth and development in edible fungi, the structure, the sequence characters and the expression profile of a GST gene vv-gto1 of Volvariella volvacea were analyzed.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>ZOOM software was used to map sequencing read (reads) from genome and transcriptome against the splicing sequence of genome, to confirm the complete length and the accuracy of the gene sequence, and to visualize gene structure. The MEGA 5.1 was used to do the multiple sequence alignment and phylogenetic tree analysis. Real time fluorescent quantitative PCR was used to determine the expression levels of vv-gtol at different growth periods of Volvariella volvacea.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>The full sequence of vv-gtol covered 2083 bp, containing 11 exons and 10 introns, and encoded a protein with 356 amino acids. 5'UTR was 305 bp which contains one intron region, and 3'UTR was 86bp. Two intron retentions could be recognized during RNA processing, and the transcripts formed by the intron retention could not translate the correct conservative functional domains. The full-length of vv-gtol had more than 50 accurate positioning genome sequencing reads, suggesting that genome sequencing and assembly results are accurate and reliable. The phylogenetic tree showed that GTO1 of Volvariella volvacea belonged to the subclass I of the Omega class of glutathione S-transferase superfamily, and had the closest relationship with GTO1 and GTO2 in Phanerochaete chrysosporium. The analysis of digital gene expression profiling, fluorescence quantitative PCR and proteomics showed that vv-gtol had the highest expression level in the heterokaryotic hyphae.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>This is the first time to obtain a gene encoding glutathione S-transferase from Volvariella volvacea which belongs to Omega class. Our study showed that the gene may play an important role during the special biological functions of heterokaryotic hyphae. This study also suggested that Volvariella volvacea heterokaryotic hyphae in H1521 had stronger resistance ability than other samples. In addition, vv-gto1 could form different alternative splicesome to regulate gene transcription and translation, and ultimately affect the function of the protein.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25803894</PMID><DateCompleted><Year>2015</Year><Month>05</Month><Day>08</Day></DateCompleted><DateRevised><Year>2015</Year><Month>03</Month><Day>25</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0001-6209</ISSN><JournalIssue CitedMedium="Print"><Volume>54</Volume><Issue>10</Issue><PubDate><Year>2014</Year><Month>Oct</Month><Day>04</Day></PubDate></JournalIssue><Title>Wei sheng wu xue bao = Acta microbiologica Sinica</Title><ISOAbbreviation>Wei Sheng Wu Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Sequence characterization and differential expression of a glutathione S-transferase gene vv-gto1 from Volvariella volvacea].</ArticleTitle><Pagination><MedlinePgn>1171-7</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">Based on the analysis of omics data of Volvariella volvacea, a gene encoding glutathione S- transferase (GSTs) named vv-gtol was obtained. To reveal the role of GSTs in the growth and development in edible fungi, the structure, the sequence characters and the expression profile of a GST gene vv-gto1 of Volvariella volvacea were analyzed.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">ZOOM software was used to map sequencing read (reads) from genome and transcriptome against the splicing sequence of genome, to confirm the complete length and the accuracy of the gene sequence, and to visualize gene structure. The MEGA 5.1 was used to do the multiple sequence alignment and phylogenetic tree analysis. Real time fluorescent quantitative PCR was used to determine the expression levels of vv-gtol at different growth periods of Volvariella volvacea.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The full sequence of vv-gtol covered 2083 bp, containing 11 exons and 10 introns, and encoded a protein with 356 amino acids. 5'UTR was 305 bp which contains one intron region, and 3'UTR was 86bp. Two intron retentions could be recognized during RNA processing, and the transcripts formed by the intron retention could not translate the correct conservative functional domains. The full-length of vv-gtol had more than 50 accurate positioning genome sequencing reads, suggesting that genome sequencing and assembly results are accurate and reliable. The phylogenetic tree showed that GTO1 of Volvariella volvacea belonged to the subclass I of the Omega class of glutathione S-transferase superfamily, and had the closest relationship with GTO1 and GTO2 in Phanerochaete chrysosporium. The analysis of digital gene expression profiling, fluorescence quantitative PCR and proteomics showed that vv-gtol had the highest expression level in the heterokaryotic hyphae.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">This is the first time to obtain a gene encoding glutathione S-transferase from Volvariella volvacea which belongs to Omega class. Our study showed that the gene may play an important role during the special biological functions of heterokaryotic hyphae. This study also suggested that Volvariella volvacea heterokaryotic hyphae in H1521 had stronger resistance ability than other samples. In addition, vv-gto1 could form different alternative splicesome to regulate gene transcription and translation, and ultimately affect the function of the protein.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Junjie</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Lixian</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Jingjing</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Xie</LastName><ForeName>Baogui</ForeName><Initials>B</Initials></Author></AuthorList><Language>chi</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>KF996272</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Wei Sheng Wu Xue Bao</MedlineTA><NlmUniqueID>21610860R</NlmUniqueID><ISSNLinking>0001-6209</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.5.1.18</RegistryNumber><NameOfSubstance UI="D005982">Glutathione Transferase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005091" MajorTopicYN="N">Exons</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005982" MajorTopicYN="N">Glutathione Transferase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007438" MajorTopicYN="N">Introns</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017421" MajorTopicYN="N">Sequence Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055367" MajorTopicYN="N">Volvariella</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>3</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>3</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>5</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25803894</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Guo, Lixian" sort="Guo, Lixian" uniqKey="Guo L" first="Lixian" last="Guo">Lixian Guo</name><name sortKey="Xie, Baogui" sort="Xie, Baogui" uniqKey="Xie B" first="Baogui" last="Xie">Baogui Xie</name><name sortKey="Yan, Junjie" sort="Yan, Junjie" uniqKey="Yan J" first="Junjie" last="Yan">Junjie Yan</name><name sortKey="Zhao, Jingjing" sort="Zhao, Jingjing" uniqKey="Zhao J" first="Jingjing" last="Zhao">Jingjing Zhao</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhanerochaeteV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000290 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000290 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhanerochaeteV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:25803894
|texte= [Sequence characterization and differential expression of a glutathione S-transferase gene vv-gto1 from Volvariella volvacea].
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:25803894" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhanerochaeteV1
| This area was generated with Dilib version V0.6.37. |  |